P-89: The Developmental Rates of Early Mouse Embryos Derived from In Vitro Fertilization of Mature Oocytes Obtained from Ovarian Stimulation Using A Combination of HMG and Estradiol Valerate
نویسندگان
چکیده مقاله:
Background: In recent years, a combination of hormones and agents in assisted reproductive technology (ART) cycles as a means of stimulating the ovary and developing more follicles. With respect to the positive effects of Estradiol Valerate on reproductive tissues and the impact on native genes of the egg that transferred to the embryo after fertilization, it seems that this agent can also effective on embryo developmental stages in combination of gonadotropin human menopausal (HMG). Materials and Methods: 40 NMRI female mice were divided into two groups. Control group received HMG alone (10 IU/mouse) and the estradiol-treated group receiving the combination of HMG and estradiol valerate (1 μg/mouse). After induction of ovulation by HCG, MII oocytes collected from the above groups and then were transferred to fertilization medium for IVF. Finally, the resulting development rates of embryos to the blastocyst stage were evaluated. Results: The results showed that the mean number of eggs obtained in the control and estradiol-treated groups were 20.6 ± 12.46 and 19.56 ± 8.84 and also the mean percentage of healthy mature oocytes were 57 ± 26 and 60 ± 17 respectively that the difference between the two groups was not statistically significant in these respects(p=0.698, p=0.614). Fertilized egg and blastocyst rates obtained in the control group and estradioltreated respectively were 30% ± 21 vs. 36% ± 21 and 49% ± 26 vs. 61% ± 31 that also the difference was not significant in these cases (p=0.713 and p=0.232). Conclusion: This study showed that administration of estradiol in combination of HMG as ovarian stimulator has no any significant effect on oocyte quality and embryo development.
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عنوان ژورنال
دوره 6 شماره 2
صفحات -
تاریخ انتشار 2012-09-01
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